Publications

See here for a complete list of publications.

2022-2024

Hirata, E., Sakata, K., Dearden, G.I., Menon, I., Chiduza, G.N., Menon, A.K. Molecular characterization of Rft1, a membrane protein associated with congenital disorder of glycosylation type 1N. 2024. bioRxiv doi: https://doi.org/10.1101/2024.04.03.587922

Menon, I., Sych, T., Son, Y., Morizumi, T., Lee, J., Ernst, O.P., Khelashvili, G., Sezgin, E.,Levitz, J., Menon, A.K. A cholesterol switch controls phospholipid scrambling by G protein-coupled receptors. 2024. J. Biol. Chem. https://www.jbc.org/article/S0021-9258(24)00025-5/fulltext

Bartoš, L., Menon, A.K, Vácha, R. Insertases Scramble Lipids: Molecular Simulations of MTCH2. 2024. Structure 32, 505-510. https://doi.org/10.1016/j.str.2024.01.012

Jahn, H., Bartoš, L., Dearden, G.I., Dittman, J.S., Holthuis, J.C.M., Vácha, R., Menon, A.K. Phospholipids are imported into mitochondria by VDAC, a dimeric beta barrel scramblase. 2023. Nature Communications 14, 8115 https://www.nature.com/articles/s41467-023-43570-y

Holthuis, J.C.M., Jahn, H., Menon, A.K., Mizushima, N. An alliance between lipid transfer proteins and scramblases for membrane expansion. 2022. https://doi.org/10.12703/r-01-0000015 

Sarina Veit, Laura Charlotte Paweletz, Søren S.-R. Bohr, Anant K Menon, Nikos S. Hatzakis, Thomas Guenther Pomorski. A single vesicle fluorescence-bleaching assay for multi-parameter analysis of proteoliposomes by total internal reflection fluorescence microscopy. 2022. ACS Applied Materials & Interfaces, 14, 29659 https://pubs.acs.org/doi/10.1021/acsami.2c07454

Morra, G., Razavi, A.M., Menon, A.K., Khelashvili, G. Cholesterol occupies the lipid translocation pathway to block phospholipid scrambling by a G protein-coupled receptor. 2022. Structure https://www.sciencedirect.com/science/article/pii/S0969212622001848

Feuda, R., Menon, A.K., Göpfert, M.C. Rethinking opsins. Molecular Biology and Evolution 2022 39: https://academic.oup.com/mbe/article/39/3/msac033/6526402

Khelashvili, G., Menon, A.K. Phospholipid Scrambling by G Protein–Coupled Receptors. Annu. Rev. Biophys. 2022 http://www.annualreviews.org/eprint/QQZ6G3ACDN463MZAADT5/full/10.1146/annurev-biophys-090821-083030

Yicheng Wang, Anant K. Menon, Yuta Maki, Yi-Shi Liu, Yugo Iwasaki, Morihisa Fujita, Paula A. Guerrero, Daniel Varón Silva, Peter H. Seeberger, Yoshiko Murakami, Taroh Kinoshita. Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis. PNAS 2022 119:e2115083119 (https://doi.org/10.1073/pnas.2115083119)

Cowton, A., Bütikofer, P., Häner, R., Menon, A.K. Identification of TbPBN1 in Trypanosoma brucei reveals a conserved heterodimeric architecture for glycosylphosphatidylinositol-mannosyltransferase I. Mol Microbiol. 2022 Feb;117(2):450-461

2021

Jenni, A, Knüsel, S, Nagar, R, Benninger, M, Häner, R, Ferguson, MAJ, Roditi, I, Menon, AK, Bütikofer, P. Elimination of GPI2 suppresses glycosylphosphatidylinositol GlcNAc transferase activity and alters GPI glycan modification in Trypanosoma brucei. J. Biol. Chem. 2021 Aug;297(2):100977. doi: 10.1016/j.jbc.2021.100977.

Mathiassen, P.P.M., Menon, A.K., Günther Pomorski, T. Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter. Sci Rep 11, 14364 (2021). https://doi.org/10.1038/s41598-021-93664-0

Khelashvili, G, Pillai, AN, Lee, J, Pandey, K, Payne, AM, Siegel, Z, Cuendet, MA, Lewis, TR, Arshavsky, VY, Broichhagen, J, Levitz, J, Menon, AK. Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin. Sci Rep. 2021 May 18;11(1):10536. doi: 10.1038/s41598-021-90039-3.PMID: 34006992

Verchère A, Cowton A, Jenni, A, Rauch M, Häner R, Graumann, J, Bütikofer P, Menon AK. Complexity of the eukaryotic dolichol-linked oligosaccharide scramblase suggested by activity correlation profiling mass spectrometry. Sci Rep. 2021 Jan 14;11(1):1411. doi: 10.1038/s41598-020-80956-0.PMID: 33446867

2019-2020

Picca G, Probst M, Langenegger SM, Khorev O, Bütikofer P, Menon AK, Häner R. Nonenzymatic synthesis of anomerically pure, mannosyl-based molecular probes for scramblase identification studies. Beilstein J. Org. Chem. 2020, 16, 1732-1739.

Chauhan N, Sere YY, Sokol AM, Graumann J, Menon AK. A PhotoClick cholesterol-based quantitative proteomics screen for cytoplasmic sterol binding proteins in Saccharomyces cerevisiae. Yeast. 2020 Jan;37(1):15-25. doi: 10.1002/yea.3448.

Khelashvili G, Chauhan N, Pandey K, Eliezer D, Menon AK. Exchange of water for sterol underlies sterol egress from a StARkin domain. Elife. 2019 eLife 2019;8:e53444 DOI: 10.7554/eLife.53444

Chauhan N, Jentsch JA, Menon AK. Measurement of intracellular sterol transport in yeast. Methods Mol Biol. 2019;1949:115-136. doi: 10.1007/978-1-4939-9136-5_10. PMID: 30790253

Chandra G, Defour A, Mamchoui K, Pandey K, Mishra S, Mouly V, Sreetama S, Mahad Ahmad M, Mahjneh I, Morizono H, Pattabiraman N, Menon AK, Jaiswal JK. Dysregulated calcium homeostasis prevents plasma membrane repair in Anoctamin 5/TMEM16E-deficient patient muscle cells. Cell Death Discov. 2019 Jul 18;5:118. doi: 10.1038/s41420-019-0197-z. eCollection 2019. PMID: 31341644

2018

Wang L, Iwasaki Y, Andra KK, Pandey K, Menon AK, Bütikofer P. Scrambling of natural and fluorescently tagged phosphatidylinositol by reconstituted G protein-coupled receptor and TMEM16 scramblases. J Biol Chem. 2018 Nov 23;293(47):18318-18327. doi: 10.1074/jbc.RA118.004213. Epub 2018 Oct 4.

Roelants FM, Chauhan N, Muir A, Davis JC, Menon AK, Levine TP, Thorner J. TOR complex 2-regulated protein kinase Ypk1 controls sterol distribution by inhibiting StARkin domain-containing proteins located at plasma membrane-endoplasmic reticulum contact sites. Mol Biol Cell. 2018 Aug 15;29(17):2128-2136. doi: 10.1091/mbc.E18-04-0229. Epub 2018 Jun 21. PMID: 29927351

Andra KK, Dorsey S, Royer CA, Menon AK. Structural mapping of fluorescently-tagged, functional nhTMEM16 scramblase in a lipid bilayer. J Biol Chem. 2018 Aug 3;293(31):12248-12258. doi: 10.1074/jbc.RA118.003648. Epub 2018 Jun 14. PMID: 29903908

Menon AK. Sterol gradients in cells. Curr Opin Cell Biol. 2018 Aug;53:37-43. doi: 10.1016/j.ceb.2018.04.012. Epub 2018 May 18. Review. PMID: 29783105

Kobayashi T, Menon AK. Transbilayer lipid asymmetry. Curr Biol. 2018 Apr 23;28(8):R386-R391. doi: 10.1016/j.cub.2018.01.007. Review. PMID: 29689220 

Lee BC, Khelashvili G, Falzone M, Menon AK, Weinstein H, Accardi A. Gating mechanism of the extracellular entry to the lipid pathway in a TMEM16 scramblase. Nat Commun. 2018 Aug 14;9(1):3251. doi: 10.1038/s41467-018-05724-1. PMID: 30108217

Malvezzi M, Andra KK, Pandey K, Lee BC, Falzone ME, Brown A, Iqbal R, Menon AK, Accardi A. Out-of-the-groove transport of lipids by TMEM16 and GPCR scramblases. Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):E7033-E7042. doi: 10.1073/pnas.1806721115. Epub 2018 Jun 20. PMID: 29925604

Quon E, Sere YY, Chauhan N, Johansen J, Sullivan DP, Dittman JS, Rice WJ, Chan RB, Di Paolo G, Beh CT, Menon AK. Endoplasmic reticulum-plasma membrane contact sites integrate sterol and phospholipid regulation. PLoS Biol. 2018 May 21;16(5):e2003864. doi: 10.1371/journal.pbio.2003864. eCollection 2018 May. PMID: 29782498

Jentsch JA, Kiburu I, Pandey K, Timme M, Ramlall T, Levkau B, Wu J, Eliezer D, Boudker O, Menon AK. Structural basis of sterol binding and transport by a yeast StARkin domain. J Biol Chem. 2018 Apr 13;293(15):5522-5531. doi: 10.1074/jbc.RA118.001881. Epub 2018 Feb 20. PMID: 29463678 

Morra G, Razavi AM, Pandey K, Weinstein H, Menon AK, Khelashvili G. Mechanisms of lipid scrambling by the G protein-coupled receptor opsin. Structure. 2018 Feb 6;26(2):356-367.e3. doi: 10.1016/j.str.2017.11.020. Epub 2017 Dec 28. PMID: 29290486

Solanko LM, Sullivan DP, Sere YY, Szomek M, Lunding A, Solanko KA, Pizovic A, Stanchev LD, Pomorski TG, Menon AK, Wüstner D. Ergosterol is mainly located in the cytoplasmic leaflet of the yeast plasma membrane. Traffic. 2018 Mar;19(3):198-214. doi: 10.1111/tra.12545. PMID: 29282820

2017

Pandey K, Ploier B, Goren MA, Levitz J, Khelashvili G, Menon AK. An engineered opsin monomer scrambles phospholipids. Sci Rep. 2017 Dec 1;7(1):16741. doi: 10.1038/s41598-017-16842-z. PMID: 29196630

Verchère A, Ou WL, Ploier B, Morizumi T, Goren MA, Bütikofer P, Ernst OP, Khelashvili G, Menon AK. Light-independent phospholipid scramblase activity of bacteriorhodopsin from Halobacterium salinarum. Sci Rep. 2017 Aug 25;7(1):9522. doi: 10.1038/s41598-017-09835-5. PMID: 28842688

Yi L, Bozkurt G, Li Q, Lo S, Menon AK, Wu H. Disulfide bond formation and N-glycosylation modulate protein-protein interactions in GPI-Transamidase (GPIT). Sci Rep. 2017 Apr 4;8:45912. doi: 10.1038/srep45912. PMID: 28374821

Cotton RJ, Ploier B, Goren MA, Menon AK, Graumann J. flippant-An R package for the automated analysis of fluorescence-based scramblase assays. BMC Bioinformatics. 2017 Mar 3;18(1):146. doi: 10.1186/s12859-017-1542-y. PMID: 28253836

Dittman JS, Menon AK. Speed limits for nonvesicular intracellular sterol transport. Trends Biochem Sci. 2017 Feb;42(2):90-97. doi: 10.1016/j.tibs.2016.11.004. Epub 2016 Dec 10. Review. PMID: 27956059

Gottier P, Gonzalez-Salgado A, Menon AK, Liu YC, Acosta-Serrano A, Bütikofer P. RFT1 protein affects glycosylphosphatidylinositol (GPI) anchor glycosylation. J Biol Chem. 2017 Jan 20;292(3):1103-1111. doi: 10.1074/jbc.M116.758367. Epub 2016 Dec 7. PMID: 27927990

Skrabanek, LA, Menon AK. Phylogenetic profiling identifies glucosyl-phosphoryl dolichol scramblase candidates. bioRxiv doi: https://doi.org/10.1101/209106

2016

Pomorski TG, Menon AK. Lipid somersaults: Uncovering the mechanisms of protein-mediated lipid flipping. Prog Lipid Res. 2016 Oct;64:69-84. doi: 10.1016/j.plipres.2016.08.003. Epub 2016 Aug 12. Review. PMID: 27528189

Lee BC, Menon AK, Accardi A. The nhTMEM16 scramblase is also a nonselective ion channel. Biophys J. 2016 Nov 1;111(9):1919-1924. doi: 10.1016/j.bpj.2016.09.032. PMID: 27806273

Ploier B, Caro LN, Morizumi T, Pandey K, Pearring JN, Goren MA, Finnemann SC, Graumann J, Arshavsky VY, Dittman JS, Ernst OP, Menon AK. Dimerization deficiency of enigmatic retinitis pigmentosa-linked rhodopsin mutants. Nat Commun. 2016 Oct 3;7:12832. doi: 10.1038/ncomms12832. PMID: 27694816

Ploier B, Menon AK. A fluorescence-based assay of phospholipid scramblase activity. J Vis Exp. 2016 Sep 20;(115). doi: 10.3791/54635. PMID: 27684510

Menon AK, Levine TP. The cellular lipid landscape. Biochim Biophys Acta. 2016 Aug;1861(8 Pt B):755-756. doi: 10.1016/j.bbalip.2016.05.004. Epub 2016 May 11. PMID: 27179417

Chauhan N, Farine L, Pandey K, Menon AK, Bütikofer P. Lipid topogenesis - 35 years on. Biochim Biophys Acta. 2016 Aug;1861(8 Pt B):757-766. doi: 10.1016/j.bbalip.2016.02.025. Epub 2016 Mar 2. Review. PMID: 26946259

2015

Hankins HM, Sere YY, Diab NS, Menon AK, Graham TR. Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles. Mol Biol Cell. 2015 Dec 15;26(25):4674-85. doi: 10.1091/mbc.E15-07-0487. Epub 2015 Oct 14. PMID: 26466678

Menon AK, Levine TP. Cell biology: Countercurrents in lipid flow. Nature. 2015 Sep 10;525(7568):191-2. doi: 10.1038/525191a. PMID: 26354476

Verchère A, Menon AK. Structural biology: Lipid gymnastics. Nature. 2015 Aug 27;524(7566):420-2. doi: 10.1038/nature15202. Epub 2015 Aug 12. PMID: 26266983

Ernst OP, Menon AK. Phospholipid scrambling by rhodopsin. Photochem Photobiol Sci. 2015 Nov;14(11):1922-31. doi: 10.1039/c5pp00195a. Review. PMID: 26179029

Gatta AT, Wong LH, Sere YY, Calderón-Noreña DM, Cockcroft S, Menon AK, Levine TP. A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport. Elife. 2015 May 22;4. doi: 10.7554/eLife.07253. PMID: 26001273

2014 and earlier

Goren MA, Morizumi T, Menon I, Joseph JS, Dittman JS, Cherezov V, Stevens RC, Ernst OP, Menon AK. Constitutive phospholipid scramblase activity of a G protein-coupled receptor. Nat Commun. 2014 Oct 8;5:5115. doi: 10.1038/ncomms6115. PMID: 25296113

Holthuis JC, Menon AK. Lipid landscapes and pipelines in membrane homeostasis. Nature. 2014 Jun 5;510(7503):48-57. doi: 10.1038/nature13474. Review. PMID: 24899304

Malvezzi M, Chalat M, Janjusevic R, Picollo A, Terashima H, Menon AK, Accardi A. Ca2+-dependent phospholipid scrambling by a reconstituted TMEM16 ion channel. Nat Commun. 2013;4:2367. doi: 10.1038/ncomms3367. PMID: 23996062

Levine TP, Menon AK. A protein pair with PIPs inside. Structure. 2013 Jul 2;21(7):1070-1. doi: 10.1016/j.str.2013.06.010. PMID: 23823324

Jelk J, Gao N, Serricchio M, Signorell A, Schmidt RS, Bangs JD, Acosta-Serrano A, Lehrman MA, Bütikofer P, Menon AK. Glycoprotein biosynthesis in a eukaryote lacking the membrane protein Rft1. J Biol Chem. 2013 Jul 12;288(28):20616-23. doi: 10.1074/jbc.M113.479642. Epub 2013 May 28. PMID: 23720757

Georgiev AG, Johansen J, Ramanathan VD, Sere YY, Beh CT, Menon AK. Arv1 regulates PM and ER membrane structure and homeostasis but is dispensable for intracellular sterol transport. Traffic. 2013 Aug;14(8):912-21. doi: 10.1111/tra.12082. Epub 2013 Jun 6. PMID: 23668914

Beh, CT, McMaster, CR, Kozminski, KG, Menon, AK A detour for yeast oxysterol-binding protein homologues and nonvesicular trafficking. J Biol Chem. 2012 Mar 30;287(14):11481-8. doi: 10.1074/jbc.R111.338400. Epub 2012 Feb 14. Review. PMID: 22334669

Chalat, M, Menon, I, Turan, Z, Menon, AK Reconstitution of glucosylceramide flip-flop across the endoplasmic reticulum: implications for the mechanism of glycosphingolipid biosynthesis. J Biol Chem. 2012 May 4;287(19):15523-32. doi: 10.1074/jbc.M112.343038. Epub 2012 Mar 15. PMID: 22427661

 Georgiev, A, Sullivan, DP, Kersting, MC, Dittman, JS, Beh, CT, Menon, AK Osh proteins regulate membrane sterol organization but are not required for sterol movement between the ER and PM. Traffic. 2011 Oct;12(10):1341-55. doi: 10.1111/j.1600-0854.2011.01234.x. Epub 2011 Jul 19. PMID: 21689253 

Menon, I, Huber, T, Sanyal, S, Banerjee, S, Barré, P, Canis, S, Warren, JD, Hwa, J, Sakmar, TP, Menon, AK Opsin is a phospholipid flippase. Curr Biol. 2011 Jan 25;21(2):149-53. doi: 10.1016/j.cub.2010.12.031. Epub 2011 Jan 13. PMID: 21236677

Sanyal, S, Menon, AK Stereoselective transbilayer translocation of mannosyl phosphoryl dolichol by an endoplasmic reticulum flippase. Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11289-94. doi: 10.1073/pnas.1002408107. Epub 2010 Jun 7. PMID: 20534553

Sanyal S, Menon AK. Flipping lipids: why an' what's the reason for? ACS Chem Biol. 2009 Nov 20;4(11):895-909. doi: 10.1021/cb900163d. Review. PMID: 19689162 

Sanyal S, Menon AK. Specific transbilayer translocation of dolichol-linked oligosaccharides by an endoplasmic reticulum flippase. Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):767-72. doi: 10.1073/pnas.0810225106. Epub 2009 Jan 7. PMID: 19129492